Prevalence of antimicrobial resistance in Escherichia coli isolated from patients with gastroenteritis

Authors

DOI:

https://doi.org/10.52138/sitec.v13i1.314

Keywords:

Antimicrobial, Genes, Inflammation, Gastrointestinal tract

Abstract

The focus of this study is to highlight the prevalence of genotypic and phenotypic factors that cause resistance in isolated bacteria from gastroenteritis patients. This is achieved by extracting DNA, detecting resistance genes and performing Antimicrobial Susceptibility Testing (AST). A total of 40 samples were analyzed, of which 7.5% tested positive for the CTX-M-1 and CTX-M-2 genes. Regarding AST, 100% of the samples were resistant to Tetracycline, Gentamicin, and Ciprofloxacin; 97.5% were resistant to Cefotaxime, 92.5% were resistant to Ampicillin, and 75% were resistant to Amoxicillin with Clavulanic Acid. Despite a high resistance index, cases of sensitivity in some samples were observed. The findings underscore the alarming state of antimicrobial resistance. Therefore, society should reconsider its habits regarding drug consumption, which presents a viable alternative to prevent the emergence of new multi-resistant bacteria.

References

BAUER, A. W.; KIRBY, W. M.; SHERRIS, J. C.; TURCK, M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, v. 45, n. 4, p. 493-496, 1966.

BRANDT, K. G.; ANTUNES, M. M. C.; SILVA, G. A. P. Diarreia aguda: manejo baseado em evidências. Journal of Pediatry, v. 91, n. 6, p. 36-43, 2015. Disponível em: https://www.sciencedirect.com/science/article/pii/S0021755715001205?via%3Dihub. Acesso em: 20 ago. 2023.

CHRISTAKI, E.; MARCOU, M.; TOFARIDES, A. Antimicrobial Resistance in Bacteria: Mechanisms, Evolution, and Persistence. Journal of Molecular Evolution, v. 88, p. 26-40, 2019. Disponível em: https://sci-hub.se/https://link.springer.com/article/10.1007%2Fs00239-019-09914-3. Acesso em: 19 ago. 2023.

CLINICAL AND LABORATORY STANDARDS INSTITUTE (CLSI). Performance Standards for Antimicrobial Susceptibility Testing: 33th ed. CLSI Supplement M100. 33rd ed. Pennsylvania: Wayne, 2023.

ELLIOT, E. J. Acute gastroenteritis in children. BJM, v. 334, p.35-40, 2007. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764079/. Acesso em: 01 ago. 2023.

HUTCHINGS, M.I., TRUMAN, A.W., WALKINSON, B. Antibiotics: past, present and future. Current Opinion in Microbiology, v.51, p. 72-80, 2019. Disponível em: https://www.sciencedirect.com/science/article/pii/S1369527419300190?via%3Dihub#fig0005. Acesso em: 13 nov. 2022.

KESKIMAKI, M; EKLUND, M; PESONEN, H; HEISKANEN, T; SIITONEN, A; EPEC, EAEC and STEC in stool specimens: prevalence and molecular epidemiology of isolates. Diagnostic Microbiology and Infectious Disease, v. 40, n. 4, p. 151-156, 2001.

KLEIN, T. C. R. Bactérias Multirresistentes em um hospital do Sul do Brasil: estudo transversal analítico de 2012 a 2019. 2022. Tese (Doutorado em Enfermagem) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Florianópolis, 2022. Disponível em: https://repositorio.ufsc.br/bitstream/handle/123456789/231238/PNFR1231-T.pdf?sequence=-1&isAllowed=y. Acesso em: 13 jan. 2023.

MACEDO, M.L.A.P., CARTAXO, R.S., ALMEIDA, T. C. C., et al. Mecanismos de resistência e detecção das beta-lactamases. Unopar Científica, Ciências Biológicas e da Saúde, Londrina, vol.7, n.1, p. 59-63, 2005.

MARTINS, A. S. Teste rápido de susceptibilidade aos antimicrobianos pelo método de disco difusão direto do frasco de hemocultura, usando os pontos de corte estabelecidos pelo EUCAST. 2019. Dissertação (Mestrado em Ciências Médicas) – Universidade Federal do Rio Grande do Sul, Porto Alegre, 2019. Disponível em: https://bdigital.ufp.pt/bitstream/10284/4412/1/PPG_21378.pdf. Acesso em: 14 jan. 2023.

SATTAR, S. B. A.; SINGH, S. Bacterial Gastroenteritis. StatPearls [Internet], Treasure Island (FL), 2023. Disponível em: https://www.ncbi.nlm.nih.gov/books/NBK513295/. Acesso em: 02 nov. 2022.

TORRES, R. N. S., DREHER, A., SIMIONI, T. A. Uso de antibióticos como promotor de crescimento e seus possíveis substitutos ao seu uso em frangos de corte: aditivos, alternativas, crescimento. Nutritime Revista Eletrônica, v. 12, n. 6, 2015. Disponível em: https://www.nutritime.com.br/site/wp-content/uploads/2020/02/Artigo-336.pdf. Acesso em: 10 dez. 2022.

Published

2023-12-12

How to Cite

Mariano Silva, L. A., Vaciloto e Gonçalves, V., & Vedovelli Cardozo, M. (2023). Prevalence of antimicrobial resistance in Escherichia coli isolated from patients with gastroenteritis. Simpósio De Tecnologia Fatec Jaboticabal, 13(1), e1314. https://doi.org/10.52138/sitec.v13i1.314

Issue

Section

Simpósio - Multidisciplinar ou outras áreas